gatan digitalmicrograph 3 5 software Search Results


14 bit grayscale gatan digital micrograph 3  (Gatan Inc)
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Gatan Inc 14 bit grayscale gatan digital micrograph 3
14 Bit Grayscale Gatan Digital Micrograph 3, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ep02mp mucilair bronchial hae epithelix sarl ref  (Epithelix)
86
Epithelix ep02mp mucilair bronchial hae epithelix sarl ref
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celltiter 96 aqueous one solution cell proliferation assay  (Promega)
90
Promega celltiter 96 aqueous one solution cell proliferation assay
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paper data s1 experimental models  (Bruker Corporation)
99
Bruker Corporation paper data s1 experimental models
Figure 2. Ultrastructure of SARS-CoV-2-Infected Nasal and Bronchial Reconstituted <t>HAE</t> <t>MucilAir</t> HAE were infected on the apical surface with SARS-CoV-2 (MOI 0.1). Forty-eight hours post-inoculation, HAE were fixed and processed for transmission electron microscopy analysis, as described in Method Details. (A and B) Section of apical ciliated (Ci) and basal (Ba) cells from nasal HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with mitochondria (m) and electron-dense accumulation of viral material (white arrow). Scale bars, 2 mm (A) and 1 mm (B). (A1) Enlargement of cytoplasmic area with smooth-walled secretory vesicles containing virions (Ve) and virus-induced DMVs (asterisk). Scale bar, 1 mm. (A2) Enlargement of ciliated cell surface showing virion clusters (V). Microvilli (mi) and transverse sections of cilia (Cil) are also observed. Scale bar, 0.05 mm. (B1) Enlargement of double-membraned spherules containing electron-dense material and pieces of double membranes interspaced among virions (V). Scale bar, 0.1 mm. (B2) Enlargement of and virons (V). The white arrows point to viral double membranes seen at high magnification (inset). Scale bar, 0.2 mm. (C and D) Section of Ci and Ba cells from bronchial HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with m and electron- dense accumulation of viral materials (white arrow). Scale bar, 1 mm. (C1) Enlargement of cytoplasmic area with spherules containing virions being formed (V), electron-dense accumulation of viral material (white arrow), and pieces of membranes (black arrowhead). Scale bar, 0.5 mm. (C2) High magnification of transverse section of virions (V) at the cell surface with Ci and mi. Their double membrane (white arrowhead) and spikes at their outer edge are visible. Scale bar, 0.1 mm. (D1) Section of Ba showing virus-induced DMVs, large electron-dense accumulation of viral materials (white arrow), and double-membrane vesicles containing virions near the plasmatic membrane. Scale bar, 1 mm. (E) Enlargements of the cytoplasmic area containing viral replication sites (double white arrows) and virions being formed (V). Scale bar, 0.1 mm. (D2) Enlargement of a double-membraned spherule containing virions (V), double-membrane vesicles, and electron-dense viral materials. Scale bar, 0.1 mm. N, nucleus; DMV, cytoplasmic double-membrane vesicles; m, mitochondria; and ds, desmosome. Representative micrographs are shown from 2 independent experiments. See also Figure <t>S1.</t>
Paper Data S1 Experimental Models, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dioleoyl sn glycero 3 phosphoethanolamine n  (Croda International Plc)
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Figure 2. Ultrastructure of SARS-CoV-2-Infected Nasal and Bronchial Reconstituted <t>HAE</t> <t>MucilAir</t> HAE were infected on the apical surface with SARS-CoV-2 (MOI 0.1). Forty-eight hours post-inoculation, HAE were fixed and processed for transmission electron microscopy analysis, as described in Method Details. (A and B) Section of apical ciliated (Ci) and basal (Ba) cells from nasal HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with mitochondria (m) and electron-dense accumulation of viral material (white arrow). Scale bars, 2 mm (A) and 1 mm (B). (A1) Enlargement of cytoplasmic area with smooth-walled secretory vesicles containing virions (Ve) and virus-induced DMVs (asterisk). Scale bar, 1 mm. (A2) Enlargement of ciliated cell surface showing virion clusters (V). Microvilli (mi) and transverse sections of cilia (Cil) are also observed. Scale bar, 0.05 mm. (B1) Enlargement of double-membraned spherules containing electron-dense material and pieces of double membranes interspaced among virions (V). Scale bar, 0.1 mm. (B2) Enlargement of and virons (V). The white arrows point to viral double membranes seen at high magnification (inset). Scale bar, 0.2 mm. (C and D) Section of Ci and Ba cells from bronchial HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with m and electron- dense accumulation of viral materials (white arrow). Scale bar, 1 mm. (C1) Enlargement of cytoplasmic area with spherules containing virions being formed (V), electron-dense accumulation of viral material (white arrow), and pieces of membranes (black arrowhead). Scale bar, 0.5 mm. (C2) High magnification of transverse section of virions (V) at the cell surface with Ci and mi. Their double membrane (white arrowhead) and spikes at their outer edge are visible. Scale bar, 0.1 mm. (D1) Section of Ba showing virus-induced DMVs, large electron-dense accumulation of viral materials (white arrow), and double-membrane vesicles containing virions near the plasmatic membrane. Scale bar, 1 mm. (E) Enlargements of the cytoplasmic area containing viral replication sites (double white arrows) and virions being formed (V). Scale bar, 0.1 mm. (D2) Enlargement of a double-membraned spherule containing virions (V), double-membrane vesicles, and electron-dense viral materials. Scale bar, 0.1 mm. N, nucleus; DMV, cytoplasmic double-membrane vesicles; m, mitochondria; and ds, desmosome. Representative micrographs are shown from 2 independent experiments. See also Figure <t>S1.</t>
Dioleoyl Sn Glycero 3 Phosphoethanolamine N, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n nervonoyl d erythro sphingosine gc avanti polar lipids  (Croda International Plc)
95
Croda International Plc n nervonoyl d erythro sphingosine gc avanti polar lipids
Figure 2. Ultrastructure of SARS-CoV-2-Infected Nasal and Bronchial Reconstituted <t>HAE</t> <t>MucilAir</t> HAE were infected on the apical surface with SARS-CoV-2 (MOI 0.1). Forty-eight hours post-inoculation, HAE were fixed and processed for transmission electron microscopy analysis, as described in Method Details. (A and B) Section of apical ciliated (Ci) and basal (Ba) cells from nasal HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with mitochondria (m) and electron-dense accumulation of viral material (white arrow). Scale bars, 2 mm (A) and 1 mm (B). (A1) Enlargement of cytoplasmic area with smooth-walled secretory vesicles containing virions (Ve) and virus-induced DMVs (asterisk). Scale bar, 1 mm. (A2) Enlargement of ciliated cell surface showing virion clusters (V). Microvilli (mi) and transverse sections of cilia (Cil) are also observed. Scale bar, 0.05 mm. (B1) Enlargement of double-membraned spherules containing electron-dense material and pieces of double membranes interspaced among virions (V). Scale bar, 0.1 mm. (B2) Enlargement of and virons (V). The white arrows point to viral double membranes seen at high magnification (inset). Scale bar, 0.2 mm. (C and D) Section of Ci and Ba cells from bronchial HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with m and electron- dense accumulation of viral materials (white arrow). Scale bar, 1 mm. (C1) Enlargement of cytoplasmic area with spherules containing virions being formed (V), electron-dense accumulation of viral material (white arrow), and pieces of membranes (black arrowhead). Scale bar, 0.5 mm. (C2) High magnification of transverse section of virions (V) at the cell surface with Ci and mi. Their double membrane (white arrowhead) and spikes at their outer edge are visible. Scale bar, 0.1 mm. (D1) Section of Ba showing virus-induced DMVs, large electron-dense accumulation of viral materials (white arrow), and double-membrane vesicles containing virions near the plasmatic membrane. Scale bar, 1 mm. (E) Enlargements of the cytoplasmic area containing viral replication sites (double white arrows) and virions being formed (V). Scale bar, 0.1 mm. (D2) Enlargement of a double-membraned spherule containing virions (V), double-membrane vesicles, and electron-dense viral materials. Scale bar, 0.1 mm. N, nucleus; DMV, cytoplasmic double-membrane vesicles; m, mitochondria; and ds, desmosome. Representative micrographs are shown from 2 independent experiments. See also Figure <t>S1.</t>
N Nervonoyl D Erythro Sphingosine Gc Avanti Polar Lipids, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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addgene 62934 software  (Addgene inc)
93
Addgene inc addgene 62934 software
Figure 2. Ultrastructure of SARS-CoV-2-Infected Nasal and Bronchial Reconstituted <t>HAE</t> <t>MucilAir</t> HAE were infected on the apical surface with SARS-CoV-2 (MOI 0.1). Forty-eight hours post-inoculation, HAE were fixed and processed for transmission electron microscopy analysis, as described in Method Details. (A and B) Section of apical ciliated (Ci) and basal (Ba) cells from nasal HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with mitochondria (m) and electron-dense accumulation of viral material (white arrow). Scale bars, 2 mm (A) and 1 mm (B). (A1) Enlargement of cytoplasmic area with smooth-walled secretory vesicles containing virions (Ve) and virus-induced DMVs (asterisk). Scale bar, 1 mm. (A2) Enlargement of ciliated cell surface showing virion clusters (V). Microvilli (mi) and transverse sections of cilia (Cil) are also observed. Scale bar, 0.05 mm. (B1) Enlargement of double-membraned spherules containing electron-dense material and pieces of double membranes interspaced among virions (V). Scale bar, 0.1 mm. (B2) Enlargement of and virons (V). The white arrows point to viral double membranes seen at high magnification (inset). Scale bar, 0.2 mm. (C and D) Section of Ci and Ba cells from bronchial HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with m and electron- dense accumulation of viral materials (white arrow). Scale bar, 1 mm. (C1) Enlargement of cytoplasmic area with spherules containing virions being formed (V), electron-dense accumulation of viral material (white arrow), and pieces of membranes (black arrowhead). Scale bar, 0.5 mm. (C2) High magnification of transverse section of virions (V) at the cell surface with Ci and mi. Their double membrane (white arrowhead) and spikes at their outer edge are visible. Scale bar, 0.1 mm. (D1) Section of Ba showing virus-induced DMVs, large electron-dense accumulation of viral materials (white arrow), and double-membrane vesicles containing virions near the plasmatic membrane. Scale bar, 1 mm. (E) Enlargements of the cytoplasmic area containing viral replication sites (double white arrows) and virions being formed (V). Scale bar, 0.1 mm. (D2) Enlargement of a double-membraned spherule containing virions (V), double-membrane vesicles, and electron-dense viral materials. Scale bar, 0.1 mm. N, nucleus; DMV, cytoplasmic double-membrane vesicles; m, mitochondria; and ds, desmosome. Representative micrographs are shown from 2 independent experiments. See also Figure <t>S1.</t>
Addgene 62934 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. Ultrastructure of SARS-CoV-2-Infected Nasal and Bronchial Reconstituted HAE MucilAir HAE were infected on the apical surface with SARS-CoV-2 (MOI 0.1). Forty-eight hours post-inoculation, HAE were fixed and processed for transmission electron microscopy analysis, as described in Method Details. (A and B) Section of apical ciliated (Ci) and basal (Ba) cells from nasal HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with mitochondria (m) and electron-dense accumulation of viral material (white arrow). Scale bars, 2 mm (A) and 1 mm (B). (A1) Enlargement of cytoplasmic area with smooth-walled secretory vesicles containing virions (Ve) and virus-induced DMVs (asterisk). Scale bar, 1 mm. (A2) Enlargement of ciliated cell surface showing virion clusters (V). Microvilli (mi) and transverse sections of cilia (Cil) are also observed. Scale bar, 0.05 mm. (B1) Enlargement of double-membraned spherules containing electron-dense material and pieces of double membranes interspaced among virions (V). Scale bar, 0.1 mm. (B2) Enlargement of and virons (V). The white arrows point to viral double membranes seen at high magnification (inset). Scale bar, 0.2 mm. (C and D) Section of Ci and Ba cells from bronchial HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with m and electron- dense accumulation of viral materials (white arrow). Scale bar, 1 mm. (C1) Enlargement of cytoplasmic area with spherules containing virions being formed (V), electron-dense accumulation of viral material (white arrow), and pieces of membranes (black arrowhead). Scale bar, 0.5 mm. (C2) High magnification of transverse section of virions (V) at the cell surface with Ci and mi. Their double membrane (white arrowhead) and spikes at their outer edge are visible. Scale bar, 0.1 mm. (D1) Section of Ba showing virus-induced DMVs, large electron-dense accumulation of viral materials (white arrow), and double-membrane vesicles containing virions near the plasmatic membrane. Scale bar, 1 mm. (E) Enlargements of the cytoplasmic area containing viral replication sites (double white arrows) and virions being formed (V). Scale bar, 0.1 mm. (D2) Enlargement of a double-membraned spherule containing virions (V), double-membrane vesicles, and electron-dense viral materials. Scale bar, 0.1 mm. N, nucleus; DMV, cytoplasmic double-membrane vesicles; m, mitochondria; and ds, desmosome. Representative micrographs are shown from 2 independent experiments. See also Figure S1.

Journal: Cell reports. Medicine

Article Title: Characterization and Treatment of SARS-CoV-2 in Nasal and Bronchial Human Airway Epithelia.

doi: 10.1016/j.xcrm.2020.100059

Figure Lengend Snippet: Figure 2. Ultrastructure of SARS-CoV-2-Infected Nasal and Bronchial Reconstituted HAE MucilAir HAE were infected on the apical surface with SARS-CoV-2 (MOI 0.1). Forty-eight hours post-inoculation, HAE were fixed and processed for transmission electron microscopy analysis, as described in Method Details. (A and B) Section of apical ciliated (Ci) and basal (Ba) cells from nasal HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with mitochondria (m) and electron-dense accumulation of viral material (white arrow). Scale bars, 2 mm (A) and 1 mm (B). (A1) Enlargement of cytoplasmic area with smooth-walled secretory vesicles containing virions (Ve) and virus-induced DMVs (asterisk). Scale bar, 1 mm. (A2) Enlargement of ciliated cell surface showing virion clusters (V). Microvilli (mi) and transverse sections of cilia (Cil) are also observed. Scale bar, 0.05 mm. (B1) Enlargement of double-membraned spherules containing electron-dense material and pieces of double membranes interspaced among virions (V). Scale bar, 0.1 mm. (B2) Enlargement of and virons (V). The white arrows point to viral double membranes seen at high magnification (inset). Scale bar, 0.2 mm. (C and D) Section of Ci and Ba cells from bronchial HAE showing numerous viral vesicles (DMVs) clustered in the perinuclear region in areas with m and electron- dense accumulation of viral materials (white arrow). Scale bar, 1 mm. (C1) Enlargement of cytoplasmic area with spherules containing virions being formed (V), electron-dense accumulation of viral material (white arrow), and pieces of membranes (black arrowhead). Scale bar, 0.5 mm. (C2) High magnification of transverse section of virions (V) at the cell surface with Ci and mi. Their double membrane (white arrowhead) and spikes at their outer edge are visible. Scale bar, 0.1 mm. (D1) Section of Ba showing virus-induced DMVs, large electron-dense accumulation of viral materials (white arrow), and double-membrane vesicles containing virions near the plasmatic membrane. Scale bar, 1 mm. (E) Enlargements of the cytoplasmic area containing viral replication sites (double white arrows) and virions being formed (V). Scale bar, 0.1 mm. (D2) Enlargement of a double-membraned spherule containing virions (V), double-membrane vesicles, and electron-dense viral materials. Scale bar, 0.1 mm. N, nucleus; DMV, cytoplasmic double-membrane vesicles; m, mitochondria; and ds, desmosome. Representative micrographs are shown from 2 independent experiments. See also Figure S1.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains SARS-CoV-2 BetaCoV/France/IDF0571/2020 This paper EPI_ISL_411218 Biological Samples SARS-CoV-2-positive human nasal swab sample Bichat Claude Bernard Hospital, Paris Lescure et al. 11 Chemicals, Peptides, and Recombinant Proteins Remdesivir Med Chem Tronica Ref: HY-104077, CAS: 1809249-37-3 (+)-cis-Diltiazem hydrochloride Sigma-Aldrich Ref: D2521, CAS: 33286-22-5 Critical Commercial Assays NanoString nCounter Custom Gene Expression panels NanoString Technologies MAN-10083-01 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Ref: G3580 Deposited Data NanoString source data This paper Data S1 Experimental Models: Cell Lines VeroE6 cells ATCC CRL-1586 MucilAir Nasal HAE Epithelix SARL Ref: EP02MP MucilAir Bronchial HAE Epithelix SARL Ref: EP01MD Oligonucleotides Forward primer HKU-ORF1b-nsp14F: 50-TGGGGYTTTACRGGTAACCT-30 Eurogentec N/A Reverse primer HKU-ORF1b-nsp14R: 50-AACRCGCTTAACAAAGCACTC-30 Eurogentec N/A Probe HKU-ORF1b-nsp141P: 50-FAMTAGTTGTGATGCWATCATGACTAG-TAMRA-30 Eurogentec N/A Software and Algorithms nSolver NanoString Technologies https://www.nanostring.com/products/ analysis-software/nsolver Genomics Suite 7 Partek https://documentation.partek.com/ DigitalMicrograph Gatan https://www.gatan.com/products/tem- analysis/gatan-microscopy-suite-software Quest Graph IC50 calculator AAT Bioquest https://www.aatbio.com/tools/ic50-calculator Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/

Techniques: Infection, Transmission Assay, Electron Microscopy, Virus, Membrane